Consists of 15 fragments in 100bp increments ranging in size from 1001,500bp and additional band at 2,072bp. If samples need to be diluted, use te or other buffer of minimal. Our pcrsizer contains ten discrete fragments ranging from 100 bp to bp in 100 bp increments with a higher intensity reference band at 500 bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This marker is ideal for the size determination of pcr products. The dna includes fragments ranging from 1001,500 base pairs. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Three reference bands at 2,000 bp, 1,500 bp, and 600 bp are included for easy.
Pcr sizer 100 bp dna ladder the norgen pcrsizer 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency. Pdf universal protocol for generating 100bp size standard for. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most poplar markers used for dna fragment size determination. Invitrogen 100 bp dna ladder 50ug invitrogen 100 bp dna. The invitrogen 100 bp dna ladder is designed for sizing and quantification of double stranded. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. The bands of the ladder contain fragments following sizes. The intensity of 500 bp is about three times than the other bands to serve as reference points.
Consists of 15 fragments in 100bp increments ranging in size from 100 1,500bp and additional band at 2,072bp. The approximate mass of dna in each band is provided 0. Our highrangerplus contains nineteen discrete fragments ranging from 100 bp to 0 bp with higher intensity reference bands at 500 bp, bp and 5000 bp. This ladder can be stained with ethidium bromide or radiolabeled using t4 dna polymerase or t4 polynucleotide kinase. View online or download invitrogen qubit 4 user manual. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. The 500 bp and 1,500 bp bands contain more dna to provide internal orientation. Ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an additional fragment at 2,072 bp. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to. Thermo scientific generuler 100 bp plus dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose and polyacrylamide gels. High range dna ladder 500 bp 10 kb blue blue ngl 500 100 5x 500 500 m 204 m224 50 bp dna ladder 3. The 100 bp plus dna ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. If samples need to be diluted, use te or other buffer of minimal ionic strength.
Download a pdf containing pricing for our full product list. Sigmas pcr 100 bp low marker is provided in a solution of 10 mm trishcl ph 8. Agarose gel analysis shows that the bands between 100 bp. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2000 bp. The invitrogen 100 bp dna ladder is designed for sizing and quantification of double stranded dna on 1% to 2% agarose gels. Agarose gel picture showing the commercial ladder lane m 1kb plus ladder invitrogen lane m1 100bp ladder neb and lane minhouse.
The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel loading dye, purple 6x, no sds. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. The 100 bp dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield 12 fragments, suitable for use as molecular weight standards for electrophoresis. The ladder is generated from pcr and restriction enzyme digestion of double stranded dna. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp. The 500 bp band with higher concentration is distinguished easily from others. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. Invitrogen trackit 100 bp dna ladder fisher scientific. This readytouse format reduces handling steps and saves time.
The tracking dyes do not obscure visualization of dna bands in ladder as the dyes run outside the limits of most dna bands in ladder. Each band contains 50ng of dna, with the 500bp band intensity increased 150ng to serve as a reference. Band 600 bp two to three times brighter than other bands in ladder. Readytoload 100 bp plus dna ladder ranging from 100 to. The following reagents are supplied with this product. Generuler 100 bp plus dna ladder thermo fisher scientific. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. New england biolabs, stratagene, fermentas and invitrogen with varied characters. Apr 15, 2008 universal protocol for generating 100bp size standard for endless usage. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for. We have 1 invitrogen qubit 4 manual available for free pdf download. The norgen highrangerplus 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency.
The invitrogen trackit 100 bp dna ladder is designed for sizing and quantification of double stranded dna on 1% to 2% agarose gels. Md121221 that are suitable as molecular weight standards for 100 bp dna ladder cat. The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, bp and 2000 bp allow for easy identification and orientation of your band at a glance. Other notes contains 10 bands, 1001,000 bp in exact 100 bp spaced ladder recombinant repeats. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of. Universal protocol for generating 100bp size standard for endless. Invitrogen trackit 100 bp dna ladder 100 applications. Can be visualized on 1% to 2% agarose gels after ethidium bromide staining.
The readytouse 100 bp plus dna ladder comes premixed with loading buffer dye and ranges between 3000 to 100 bp. It consists of 15 bluntended fragments at 100 bp increments and is suitable for sizing doublestranded dna from 100 to 1,500. Universal protocol for generating 100bp size standard for endless usage. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb.
The 100 bp dna ladder consists of individual chromatographypurified dna fragments ranging in size from 100 bp to 2,000 bp. Prepared from a plasmid containing repeats of a 100 bp dna fragment. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Schematic representation of generating 100bp ladder. Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. Trackit 100 bp dna ladder is premixed with sample buffer for readytoload convenience and room temperature storage. Pdf universal protocol for generating 100bp size standard. Unstained and fluorescent dna ladders jena bioscience. Pcr 100 bp low ladder for electrophoresis of pcr fragments. For optimal resolution, the recommended agarose gel concentration is 2. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. Identification of the preparationsubstance and of the company preparation name.
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